Viability of Bacillus popilliae after Lyophilization of Liquid Nitrogen Frozen Cells1
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چکیده
منابع مشابه
Virulence and viability of Yersinia pestis 25 years after lyophilization.
When equal volumes of 6% lactose and a broth culture of Yersinia pestis were mixed before freezing, approximately 50% of the cells survived lyophilization and reconstitution on the following day. Concomitantly, the number of viable cells per 50% lethal dose increased from about 16 to 125 organisms. On subsequent storage of the lyophilized cells under vacuum in glass ampoules at 4 degrees C for ...
متن کاملFlow cytometric assessment of Lactococcus lactis isolates viability after lyophilization
Lactococcus lactis strains tolerance to the lyophilization process is one of the criteria used during the selection of starter cultures in cheese production. The viability of L. lactis strains isolated from silage and milk after the lyophilization process was evaluated in the present study by flow cytometry and cell plate counting. The LIVE/DEAD BacLight TM kit, which contains the dyes SYTO 9 a...
متن کاملFatty acids in Bacillus larvae, Bacillus lentimorbus, and Bacillus popilliae.
The types of fatty acids produced by two strains each of Bacillus larvae, B. lentimorbus, and B. popilliae, and their distribution patterns, were studied by gas-liquid chromatography. All six organisms produced eight major fatty acids: six branched (iso-C(14), -C(15), -C(16), and -C(17), and anteiso-C(15) and -C(17)), two normal (n-C(14) and -C(16)), and two minor (n-C(15) and monounsaturated n...
متن کاملHistological examination of frozen autograft treated by liquid nitrogen removed after implantation.
BACKGROUND Several oncological sterilization methods involving autoclaving, irradiation, or pasteurization have been developed for limb reconstruction of large bone defects following tumor excision. Studies involving histological examinations of these autografts have all found that osteogenesis occurs slowly. We have used frozen autografts treated by liquid nitrogen for limb reconstruction and ...
متن کاملHistological examination of frozen autograft treated by liquid nitrogen removed 6 years after implantation.
*KURAに登録されているコンテンツの利用については,著作権法に規定されている私的使用や引用などの範囲内で行ってください。 *著作権法に規定されている私的使用や引用などの範囲を超える利用を行う場合には,著作権者の許諾を得てください。ただし,著作権者 から著作権等管理事業者(学術著作権協会,日本著作出版権管理システムなど)に権利委託されているコンテンツの利用手続については ,各著作権等管理事業者に確認してください。 Title Histological examination of frozen autograft treated by liquid nitrogen removed 6 years after implantation Author(s) Tanzawa, Yoshikazu; Tsuchiya, Hiroyuki; Yamamoto, Norio; Sakayama...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1967
ISSN: 0003-6919
DOI: 10.1128/aem.15.1.163-165.1967